g-eo system Search Results


n ethylmaleimide  (Chem Impex International)
95
Chem Impex International n ethylmaleimide
N Ethylmaleimide, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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geocas9  (Addgene inc)
91
Addgene inc geocas9
Geocas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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geo cas9 plasmid  (Addgene inc)
93
Addgene inc geo cas9 plasmid
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
Geo Cas9 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxymethyl phenylboronic acid  (Chem Impex International)
95
Chem Impex International hydroxymethyl phenylboronic acid
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
Hydroxymethyl Phenylboronic Acid, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 5furandicarboxylic acid fdca  (Chem Impex International)
95
Chem Impex International 2 5furandicarboxylic acid fdca
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
2 5furandicarboxylic Acid Fdca, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1 6 anhydroglucose levoglucosan  (Chem Impex International)
95
Chem Impex International 1 6 anhydroglucose levoglucosan
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
1 6 Anhydroglucose Levoglucosan, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cellobiose  (Chem Impex International)
95
Chem Impex International cellobiose
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
Cellobiose, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3 o methyl glucose  (Chem Impex International)
95
Chem Impex International 3 o methyl glucose
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
3 O Methyl Glucose, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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2 7 dihydroxynaphthalene  (Chem Impex International)
95
Chem Impex International 2 7 dihydroxynaphthalene
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
2 7 Dihydroxynaphthalene, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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l ribose  (Chem Impex International)
95
Chem Impex International l ribose
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
L Ribose, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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3k 1s 2 s 2 aminocyclohexanecarboxylic 1m phosphate  (Chem Impex International)
95
Chem Impex International 3k 1s 2 s 2 aminocyclohexanecarboxylic 1m phosphate
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
3k 1s 2 S 2 Aminocyclohexanecarboxylic 1m Phosphate, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5 hydroxymethylfurfural hmf  (Chem Impex International)
95
Chem Impex International 5 hydroxymethylfurfural hmf
Geo <t>Cas9</t> RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).
5 Hydroxymethylfurfural Hmf, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Geo Cas9 RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).

Journal: Bio-protocol

Article Title: Assessing Temperature-Dependent DNA Cleavage by CRISPR-Cas9

doi: 10.21769/BioProtoc.5463

Figure Lengend Snippet: Geo Cas9 RNPs incubated at 37 °C (upper) or 60 °C (lower) for 10 min, after which the ribonucleoproteins (RNPs) were used in cleavage reactions carried out at 37 °C for 30 min. The appearance of lower molecular weight bands on the gel indicates successful cleavage. RNP concentrations range from 0.1 to 0.9 μM at each temperature tested, as indicated in . The rightmost lanes of each gel marked as (0) indicate negative controls with no RNP added. Molecular weight markers (M) are 1,000, 800, 600, 400, and 200 base pairs (top to bottom).

Article Snippet: Express and purify Geo Cas9 protein in-house The Geo Cas9 plasmid was acquired from Addgene (#87700) and transformed into BL21 (DE3) cells.

Techniques: Incubation, Molecular Weight

Quantitative analysis of Geo Cas9 cleavage can be carried out according to the workflow described above. The steps in the figure correspond to those in the data analysis section. Representative snapshots of the analytical steps are provided in cartoon form. (A) The original gel image in which lanes 1–6 were loaded with 5 μL of the finished Geo Cas9 RNP/DNA cleavage reactions corresponding to 0.1–0.9 μM RNP and a negative control as described in . (B) Converted gel image by the ImageJ function Invert LUT . (C) Uncleaved DNA bands in each lane were enclosed by rectangles in the order of lanes (D) Plots were generated by applying Plot Lanes after the DNA bands were selected (only the first three lanes’ plots are shown as representatives). (E) The measuring area was closed by drawing a straight line (red) at the base of each plot peak with the Straight line tool. (F) The highlighted plot area after applying the Wand (tracing) tool by clicking inside the closed peak spot of each plot. (G) Automatically generated tab with the area measurements after applying Wand (tracing) tool in the closed peaks; the tab can be saved, and the measurements can be copied for later analysis.

Journal: Bio-protocol

Article Title: Assessing Temperature-Dependent DNA Cleavage by CRISPR-Cas9

doi: 10.21769/BioProtoc.5463

Figure Lengend Snippet: Quantitative analysis of Geo Cas9 cleavage can be carried out according to the workflow described above. The steps in the figure correspond to those in the data analysis section. Representative snapshots of the analytical steps are provided in cartoon form. (A) The original gel image in which lanes 1–6 were loaded with 5 μL of the finished Geo Cas9 RNP/DNA cleavage reactions corresponding to 0.1–0.9 μM RNP and a negative control as described in . (B) Converted gel image by the ImageJ function Invert LUT . (C) Uncleaved DNA bands in each lane were enclosed by rectangles in the order of lanes (D) Plots were generated by applying Plot Lanes after the DNA bands were selected (only the first three lanes’ plots are shown as representatives). (E) The measuring area was closed by drawing a straight line (red) at the base of each plot peak with the Straight line tool. (F) The highlighted plot area after applying the Wand (tracing) tool by clicking inside the closed peak spot of each plot. (G) Automatically generated tab with the area measurements after applying Wand (tracing) tool in the closed peaks; the tab can be saved, and the measurements can be copied for later analysis.

Article Snippet: Express and purify Geo Cas9 protein in-house The Geo Cas9 plasmid was acquired from Addgene (#87700) and transformed into BL21 (DE3) cells.

Techniques: Negative Control, Generated